Isolation and characterization of microsatellite loci from the European corn borer, Ostrinia nubilalis (HÃ1⁄4bner) (Insecta: Lepidoptera: Crambidae

Few useful microsatellites are available for population studies of the European corn borer, Ostrinia nubilalis (Hübner). An enrichment strategy was used to develop microsatellite markers for O. nubilalis, and over 500 positive clones were isolated. Seventy-five contained unique microsatellites, 10 of which were polymorphic with discernable polymerase chain reaction products. The 10 loci were surveyed for variability in 72 wild individuals from central Iowa. Five loci showed no deviation from Hardy-Weinberg proportions, and all were successfully cross-amplified in the related Asian corn borer, Ostrinia furnacalis. These loci represent a significant addition to microsatellites appropriate for population studies of O. nubilalis. Disciplines Entomology Comments This article is from Molecular Ecology Resources; 8 (2008); 409-411; doi: 10.1111/j.1471-8286.2007.01974.x Rights Works produced by employees of the U.S. Government as part of their official duties are not copyrighted within the U.S. The content of this document is not copyrighted. This article is available at Iowa State University Digital Repository: http://lib.dr.iastate.edu/ent_pubs/108 Molecular Ecology Resources (2008) 8, 409–411 doi: 10.1111/j.1471-8286.2007.01974.x © 2007 Blackwell Publishing Ltd No claim to original US government works Blackwell Publishing Ltd PERMANENT GENETIC RESOURCES Isolation and characterization of microsatellite loci from the European corn borer, Ostrinia nubilalis (Hübner) (Insecta: Lepidoptera: Crambidae) KYUNG SEOK KIM, BRAD S . COATES, RICHARD L. HELLMICH, DOUGLAS V. SUMERFORD and THOMAS W. SAPPINGTON* USDA-ARS, Corn Insects and Crop Genetics Research Unit, Genetics Laboratory, Iowa State University, Ames, IA 50011, USA